SpeciesReactivity
Mouse

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Purified Scl-70 Coated Microwells (96 wells) 6111 2. Mouse Ig?s std A (0 ng/ml), 0.750 ml 6112 3. Mouse Ig?s std B (62.5 ng/ml), 0.750 ml 6113 4. Mouse Ig?s std C (125 ng/ml), 0.750 ml 6 1 1 4 5. Mouse Ig?s std D (250 ng/ml), 0.750 ml 6 1 1 5 6. Mouse Ig?s std E (500 ng/ml), 0.750 ml 6 1 1 6 7. Mouse Ig?s std F (1000 ng/ml), 0.750 ml 6 1 1 7 8. Goat Anti-mouse IgG (H+L)-HRP Conjugate (100X), 0.12 ml 6 1 1 8 9. Sample Diluent (10X), 10ml SD-10 10. Wash solution (100X), 10 ml WB-100 11. TMB Substrate solution,12 ml. 8 0 0 9 1 12. Stop solution,12 ml. 8 0 1 0 1 13. Complete Instruction Manual. M - 6 1 1 0

SpCrossreactivity
Scl-70-1 coating antigen has been immunoaffinity purified from rabbit/calf thymus using anti-Scl-70-1 sepharose. Other antigens (Sm, RNP, SSA, Jo-1 and SSB) are not detected with the ELISA. Homogeneity of Scl-70 is also verified by SDS-PAGE. This kit measures anti-Scl70 Ig's (IgG+A+M) in mouse serum. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.